human foreskin fibroblast cell line  (ATCC)

Journal: Journal of Microbiology and Biotechnology

Article Title: Upcycling Fermented Adlay Bran Ethanol Extract Residues Promotes Human Dermal Fibroblast Proliferation and Wound Healing

doi: 10.4014/jmb.2511.11014

Figure Lengend Snippet: Hs68 cells were treated with different concentrations of FRA (0– 1,600 μg/ml) and its fractions (0–200 μg/ml) for 24 h and then cytotoxicity was performed ( n = 6). Data are presented as mean ± standard error ( n = 3). Significant differences were analyzed using one-way ANOVA and Dunnett's test. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the untreated group.

Article Snippet: Human dermal fibroblast cells from the Hs68 cell line (American Type Culture Collection, USA) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin in a humidified atmosphere of 5% CO 2 and 95% air at 37°C.

Techniques:

Journal: Journal of Microbiology and Biotechnology

Article Title: Upcycling Fermented Adlay Bran Ethanol Extract Residues Promotes Human Dermal Fibroblast Proliferation and Wound Healing

doi: 10.4014/jmb.2511.11014

Figure Lengend Snippet: ( A ) The area and speed of Proliferation of Hs68 cells was measured through a 500 μm cell-free gap created by a culture insert placed in the center of a 35 mm μ-Dish (Ibidi, Germany). Measurements were made using a microscope every 6 h during 24-h incubation through real-time monitoring. Scale bar = 200 μm. ( B ) The wound area of Hs68 cells treated with 200 ppm of FRA-Bu was significantly narrowed compared to untreated cells. ( C ) The migration area expressed as a percentage based on the initial cell-free interval, increased significantly at 18 h of culture. The area area was measured in real-time using image j. Significant differences were analyzed using by unpaired t -test ( n = 3). Data are presented as mean ± standard error ( n = 3). * p < 0.05 compared with the untreated group.

Article Snippet: Human dermal fibroblast cells from the Hs68 cell line (American Type Culture Collection, USA) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin in a humidified atmosphere of 5% CO 2 and 95% air at 37°C.

Techniques: Microscopy, Incubation, Migration

Journal: Journal of Microbiology and Biotechnology

Article Title: Upcycling Fermented Adlay Bran Ethanol Extract Residues Promotes Human Dermal Fibroblast Proliferation and Wound Healing

doi: 10.4014/jmb.2511.11014

Figure Lengend Snippet: The cell ratio in the G0/G1, S, and G2/M phases of proliferation on the FRA-Bu treated Hs68 cells was analyzed using Muse Cell Cycle Analyser. ( A ) A histogram of a representative experiment shows the effect of FRA-Bu on the cell cycle profile. ( B-C ) In particular, the treated cells showed a significant increase in the S phase of the cell cycle, the origin of DNA replication, after 18 hours of culture. Significant differences were analyzed using an unpaired t -test ( n = 3). Data are presented as mean ± standard error ( n = 3). * p < 0.05 compared to the untreated group.

Article Snippet: Human dermal fibroblast cells from the Hs68 cell line (American Type Culture Collection, USA) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin in a humidified atmosphere of 5% CO 2 and 95% air at 37°C.

Techniques:

Journal: Journal of Microbiology and Biotechnology

Article Title: Upcycling Fermented Adlay Bran Ethanol Extract Residues Promotes Human Dermal Fibroblast Proliferation and Wound Healing

doi: 10.4014/jmb.2511.11014

Figure Lengend Snippet: The mRNA expressions of CCND1 , CDK4, CCND2, CDK2, CCNA1 , CCNA2 , and CCNB2 were measured by qPCR in Hs68 cells (treated or nontreated) after 18 and 24 h. Significant differences were analyzed using an unpaired t -test ( n = 3). Data are presented as mean ± standard error ( n = 3). * p < 0.05 compared to the untreated group.

Article Snippet: Human dermal fibroblast cells from the Hs68 cell line (American Type Culture Collection, USA) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin in a humidified atmosphere of 5% CO 2 and 95% air at 37°C.

Techniques:

Journal: Febs Letters

Article Title: Mitochondrial fatty acid oxidation is stimulated by red light irradiation

doi: 10.1002/1873-3468.70195

Figure Lengend Snippet: Red light stimulation of OCRs is cell‐specific. Fibroblasts (A–D) and melanocytes (E–H) were treated under similar conditions to Fig. after 2 h of irradiation at 6, 12, 36, and 150 J·cm −2 with red light (660 nm), and oxygen consumption rates (OCRs) were quantified. Basal (Panel B, F), ATP production‐linked (Panel C, G), and maximal OCRs (Panel D, H) were calculated as described in Methods. Results are expressed as means ± SD of three independent experiments; ns, nonsignificant; * P < 0.05, one‐way ANOVA followed by Dunnett's test.

Article Snippet: A human immortalized keratinocyte cell line (HaCaT, RRID:CVCL_0038 [ ]), an immortalized skin fibroblast cell line (Hs68, RRID:CVCL_0839), and a melanocyte cell line (B16F10, RRID:CVCL_0159) were acquired from ATCC, validated within the last 3 years, confirmed mycoplasma‐free every 6 months, and cultured in high‐glucose Dulbecco modified Eagle medium (DMEM) with phenol red (Gibco, Life Technologies, Waltham, MA, USA), supplemented with 10% v/v fetal bovine serum (FBS; Sigma, St. Louis, MI, USA), 110 mg·mL −1 sodium pyruvate, 4 m m l ‐glutamine, 100 U·mL −1 of penicillin, and 100 pg·mL −1 streptomycin (Gibco, Life Technologies) at pH 7.4, 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Irradiation